Transfection:Article Title: YEATS domain-containing 2 (YEATS2), targeted by microRNA miR-378a-5p, regulates growth and metastasis in head and neck squamous cell carcinoma.
Article Snippet: After transfection for 48 h, Detroit562 and FaDu cells were subjected to 10 μM EdU staining solution (KeyGEN BioTECH, China) for 2 h at 37°C. after that, the cells were fixed in 4% paraformaldehyde at room temperature for 15 min, permeabilized by 0.1% Triton X-100 at room temperature for 20 min, and incubated in Click-iT reaction mixture (KeyGEN BioTECH, China) in dark at room temperature for 30 min. 6-diamidino-2-phenylindole (DAPI, Biosharp, China) was used to stain the nuclei and the target fluorescence was visualized under fluorescence microscope at 400 × magnification.
Article Title: YEATS domain-containing 2 (YEATS2), targeted by microRNA miR-378a-5p, regulates growth and metastasis in head and neck squamous cell carcinoma
Article Snippet: After transfection for 48 h, Detroit562 and FaDu cells were subjected to 10 μM EdU staining solution (KeyGEN BioTECH, China) for 2 h at 37°C. after that, the cells were fixed in 4% paraformaldehyde at room temperature for 15 min, permeabilized by 0.1% Triton X-100 at room temperature for 20 min, and incubated in Click-iT reaction mixture (KeyGEN BioTECH, China) in dark at room temperature for 30 min. 6-diamidino-2-phenylindole (DAPI, Biosharp, China) was used to stain the nuclei and the target fluorescence was visualized under fluorescence microscope at 400 × magnification.
Staining:Article Title: YEATS domain-containing 2 (YEATS2), targeted by microRNA miR-378a-5p, regulates growth and metastasis in head and neck squamous cell carcinoma.
Article Snippet: After transfection for 48 h, Detroit562 and FaDu cells were subjected to 10 μM EdU staining solution (KeyGEN BioTECH, China) for 2 h at 37°C. after that, the cells were fixed in 4% paraformaldehyde at room temperature for 15 min, permeabilized by 0.1% Triton X-100 at room temperature for 20 min, and incubated in Click-iT reaction mixture (KeyGEN BioTECH, China) in dark at room temperature for 30 min. 6-diamidino-2-phenylindole (DAPI, Biosharp, China) was used to stain the nuclei and the target fluorescence was visualized under fluorescence microscope at 400 × magnification.
Article Title: YEATS domain-containing 2 (YEATS2), targeted by microRNA miR-378a-5p, regulates growth and metastasis in head and neck squamous cell carcinoma
Article Snippet: After transfection for 48 h, Detroit562 and FaDu cells were subjected to 10 μM EdU staining solution (KeyGEN BioTECH, China) for 2 h at 37°C. after that, the cells were fixed in 4% paraformaldehyde at room temperature for 15 min, permeabilized by 0.1% Triton X-100 at room temperature for 20 min, and incubated in Click-iT reaction mixture (KeyGEN BioTECH, China) in dark at room temperature for 30 min. 6-diamidino-2-phenylindole (DAPI, Biosharp, China) was used to stain the nuclei and the target fluorescence was visualized under fluorescence microscope at 400 × magnification.
Incubation:Article Title: YEATS domain-containing 2 (YEATS2), targeted by microRNA miR-378a-5p, regulates growth and metastasis in head and neck squamous cell carcinoma.
Article Snippet: After transfection for 48 h, Detroit562 and FaDu cells were subjected to 10 μM EdU staining solution (KeyGEN BioTECH, China) for 2 h at 37°C. after that, the cells were fixed in 4% paraformaldehyde at room temperature for 15 min, permeabilized by 0.1% Triton X-100 at room temperature for 20 min, and incubated in Click-iT reaction mixture (KeyGEN BioTECH, China) in dark at room temperature for 30 min. 6-diamidino-2-phenylindole (DAPI, Biosharp, China) was used to stain the nuclei and the target fluorescence was visualized under fluorescence microscope at 400 × magnification.
Article Title: YEATS domain-containing 2 (YEATS2), targeted by microRNA miR-378a-5p, regulates growth and metastasis in head and neck squamous cell carcinoma
Article Snippet: After transfection for 48 h, Detroit562 and FaDu cells were subjected to 10 μM EdU staining solution (KeyGEN BioTECH, China) for 2 h at 37°C. after that, the cells were fixed in 4% paraformaldehyde at room temperature for 15 min, permeabilized by 0.1% Triton X-100 at room temperature for 20 min, and incubated in Click-iT reaction mixture (KeyGEN BioTECH, China) in dark at room temperature for 30 min. 6-diamidino-2-phenylindole (DAPI, Biosharp, China) was used to stain the nuclei and the target fluorescence was visualized under fluorescence microscope at 400 × magnification.
Fluorescence:Article Title: YEATS domain-containing 2 (YEATS2), targeted by microRNA miR-378a-5p, regulates growth and metastasis in head and neck squamous cell carcinoma.
Article Snippet: After transfection for 48 h, Detroit562 and FaDu cells were subjected to 10 μM EdU staining solution (KeyGEN BioTECH, China) for 2 h at 37°C. after that, the cells were fixed in 4% paraformaldehyde at room temperature for 15 min, permeabilized by 0.1% Triton X-100 at room temperature for 20 min, and incubated in Click-iT reaction mixture (KeyGEN BioTECH, China) in dark at room temperature for 30 min. 6-diamidino-2-phenylindole (DAPI, Biosharp, China) was used to stain the nuclei and the target fluorescence was visualized under fluorescence microscope at 400 × magnification.
Article Title: YEATS domain-containing 2 (YEATS2), targeted by microRNA miR-378a-5p, regulates growth and metastasis in head and neck squamous cell carcinoma
Article Snippet: After transfection for 48 h, Detroit562 and FaDu cells were subjected to 10 μM EdU staining solution (KeyGEN BioTECH, China) for 2 h at 37°C. after that, the cells were fixed in 4% paraformaldehyde at room temperature for 15 min, permeabilized by 0.1% Triton X-100 at room temperature for 20 min, and incubated in Click-iT reaction mixture (KeyGEN BioTECH, China) in dark at room temperature for 30 min. 6-diamidino-2-phenylindole (DAPI, Biosharp, China) was used to stain the nuclei and the target fluorescence was visualized under fluorescence microscope at 400 × magnification.
Microscopy:Article Title: YEATS domain-containing 2 (YEATS2), targeted by microRNA miR-378a-5p, regulates growth and metastasis in head and neck squamous cell carcinoma.
Article Snippet: After transfection for 48 h, Detroit562 and FaDu cells were subjected to 10 μM EdU staining solution (KeyGEN BioTECH, China) for 2 h at 37°C. after that, the cells were fixed in 4% paraformaldehyde at room temperature for 15 min, permeabilized by 0.1% Triton X-100 at room temperature for 20 min, and incubated in Click-iT reaction mixture (KeyGEN BioTECH, China) in dark at room temperature for 30 min. 6-diamidino-2-phenylindole (DAPI, Biosharp, China) was used to stain the nuclei and the target fluorescence was visualized under fluorescence microscope at 400 × magnification.
Article Title: YEATS domain-containing 2 (YEATS2), targeted by microRNA miR-378a-5p, regulates growth and metastasis in head and neck squamous cell carcinoma
Article Snippet: After transfection for 48 h, Detroit562 and FaDu cells were subjected to 10 μM EdU staining solution (KeyGEN BioTECH, China) for 2 h at 37°C. after that, the cells were fixed in 4% paraformaldehyde at room temperature for 15 min, permeabilized by 0.1% Triton X-100 at room temperature for 20 min, and incubated in Click-iT reaction mixture (KeyGEN BioTECH, China) in dark at room temperature for 30 min. 6-diamidino-2-phenylindole (DAPI, Biosharp, China) was used to stain the nuclei and the target fluorescence was visualized under fluorescence microscope at 400 × magnification.
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